SubtiBank SubtiBank
crh [2019-06-28 11:37:39]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

crh [2019-06-28 11:37:39]

Carbon-flux regulating HPr, formerly known as Catabolite repression HPr-like protein, control of flux through the harmful methylglyoxal pathway, minor cofactor of the [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA] transcription factor
locus
BSU34740
pI
4.70
mw
9.19 kDa
protein length
85 aa Sequence Blast
gene length
258 bp Sequence Blast
function
control of carbon flux
product
carbon-flux regulating HPr
essential
no
synonyms
yvcM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    3,569,292 3,569,549

    The protein

    Protein family

  • [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] family

    • Paralogous protein(s)

  • [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|PtsH]

    • [SW|Domains]

  • [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|HPr] domain (185)

    • Modification

  • phosphorylation on Ser46 by [protein|771F205F818A755A661B8E1C95365A4F6AEB05C7|HprK] [Pubmed|9237995,21992469,22092971]

    • Structure

  • [PDB|2AK7] (dimeric Crh-Ser46-P) [pubmed|16411239]
  • [PDB|1ZVV] ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]-Crh-DNA complex)
  • [PDB|2RLZ] (dimer)
  • [PDB|1MU4]

    • Additional information

  • Crh does not possess the phosphorylation site used for [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] phosphotransfer (His-15 in [protein|29B793660E4D30C0656248F3EF403FEF76FB9025|PtsH]), it can only be phosphorylated on Ser-46

    • Expression and Regulation

    Operons

    genes
    [gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
    description
    [Pubmed|9237995]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16272399], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]

    • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|search|trxB]' [PubMed|20525796]
    • view in new tab

    Biological materials

    Mutant

  • GP860 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::aphA3) [Pubmed|21992469], available in [SW|Jörg Stülke]'s lab
  • QB7097 (spc), available in [SW|Jörg Stülke]'s lab
  • BKE34740 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT
  • BKK34740 (Δ[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34740 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAGATCTCCCCTTTTCT, downstream forward: _UP4_GCTTACGTTCAAGAAGAAGT

    • Expression vectors

  • pGP641 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
  • pGP734 (C-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab

    • lacZ fusion

  • see [gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]

    • two-hybrid system

  • [gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh], crh(Ser46Asp), crh(Ser46Ala) B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • labs

  • [SW|Anne Galinier], University of Marseille, France
  • [SW|Richard Brennan], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]

    • References

  • 12972249,9973552,9237995,16272399,15126459,10217795,17142398,16316990,12009882,10589728,12670692,11916384,11361076,11361074,18320329,18284240,16411239,21992469,22092971,29514981